FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n02_mb150.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n02_mb150.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37006973
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA16250.034.2463259
CGTATCA18800.030.34605462
CCGTATC19800.027.9295461
CGTCTAG45450.027.4148371
TCGCCGT22300.024.79832558
CTACGGT43300.023.92605213
TACGGTG45200.022.99777614
TTGACGG51850.022.07312831
CTAGACG50900.021.9350073
GCGATCT447200.021.79760770
TCCGCAA47300.021.68060333
CCTACGG50150.020.72777712
GACGCAC52200.020.6512176
GTGGTCG28250.020.31846254
CGATCTA303950.020.2442770
TGACGGC58200.020.02562732
CGACGAC21150.019.8589570
GTCCGCA52350.019.5891632
TAGACGC84350.019.5434514
ACGCACC53550.019.4116767
Produced by FastQC (version 0.11.5)