FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n02_mb149.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n02_mb149.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30972107
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA15300.038.66040859
CGTATCA17350.035.10117362
CCGTATC20200.029.80219561
TCGCCGT20100.029.6020658
CGTCTAG34600.025.0868051
CGACGAC19950.023.50954870
GTGGTCG27650.021.39228254
CTACGGT33800.021.33130613
GCGATCT333450.021.24526470
CGATCTA229150.020.31487570
CTAGACG39300.020.216173
GATCTCG31100.020.14457947
TTGACGG41400.019.78252831
TACGGTG36950.019.60752714
TCCGCAA38300.019.09910833
CCTACGG39900.018.1578512
ATCTCGG34550.018.13303648
TAGACGC66850.017.9580424
TGACGGC47000.017.64883632
AGCGATC452500.017.60500170
Produced by FastQC (version 0.11.5)