FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n02_mb148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n02_mb148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29847651
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA15400.041.59113759
CGACGAC27300.038.84708470
CGTATCA19950.032.1054962
CCGTATC21600.029.9770761
TCGCCGT21350.029.83617858
GCCGACC40400.026.68380770
CGTCTAG31800.025.9748551
GTGGTCG27800.023.54305854
GATCTCG30000.022.28323247
GCGATCT267750.021.43842170
GTCGCCG31450.020.58814257
GCCGTAT32000.020.34389760
CGATCTA181150.019.99771970
TTGACGG38650.019.65062131
ATCTCGG33550.019.6124348
CGGTGGT36000.018.4721452
TGGTCGC37400.018.06141755
TCTCGGT36900.017.83189849
TCGGTGG37400.017.4063451
GACGAGC1001200.017.4060370
Produced by FastQC (version 0.11.5)