FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n02_mb147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n02_mb147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25853413
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC20400.037.74579270
CGTCTAG28150.029.467171
GCCGACC27250.029.28494570
CGCCGTA6800.025.73547259
GCGATCT218400.024.79212470
TTGACGG35200.022.57091731
CTACGGT22600.021.52648713
AGCGATA13500.020.74112370
AGCGATC312950.020.52282770
CGATCTA138200.020.488870
TGACGGC39000.020.37169832
TACGGTG24700.019.696314
GACGGCT39350.019.56788633
TCCGCAA25950.019.42187333
GACGCAC27200.018.5293246
GTCCGCA26850.018.51015532
GCGATCA11350.018.19416870
TCGCCGT10100.018.0198958
CCTACGG27850.017.8455512
CTAGACG29050.017.8312423
Produced by FastQC (version 0.11.5)