FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n02_mb146.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n02_mb146.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27574912
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC20200.036.38709370
CGTCTAG29450.030.662221
GCCGACC28650.029.19797170
CTACGGT29250.027.40163213
CGCCGTA6650.026.31609559
TACGGTG30850.025.9804814
GACGCAC32800.025.182816
CTAGACG33700.024.8218423
TCCGCAA32650.024.54812433
CCTACGG33350.024.45270312
ACGCACC34350.023.7407917
TTGACGG37200.023.52139531
GCGATCT244950.022.91956370
GTCCGCA35450.022.70793532
ACGGTGA36600.022.37698615
AGCGATA14650.022.21901370
CGCACCT37150.021.9514478
TAGACGC62000.021.7337724
TGACGGC41400.021.21970732
AGCGATC348600.020.40214270
Produced by FastQC (version 0.11.5)