FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n02_mb145.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n02_mb145.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21915071
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC26600.048.94816670
GCCGACC35550.043.61533770
CGTCTAG21100.027.8674341
CTACGGT20950.026.39612813
TACGGTG21100.026.20847914
AGCGATA10550.025.87719770
GCGATCT139600.025.52333670
GACGCAC22050.025.3967156
ACGAGCC50700.025.33571670
CTAGACG24800.023.9918333
ACGCACC23750.023.5788467
TCCGCAA24400.023.38104833
CGATCTA88400.023.24132770
TCTGACC108450.023.14007270
CCTACGG24300.023.04522112
AGCGATC207500.022.434170
ACGGTGA26750.021.1962215
GTCCGCA27250.021.0641332
GCGATCA8150.021.04328570
TAGACGC46350.019.7841644
Produced by FastQC (version 0.11.5)