FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n02_mb144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n02_mb144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40656457
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA16850.044.0357959
CGTATCA20350.037.6660562
CGACGAC30900.034.43439570
CCGTATC21950.034.12314661
TCGCCGT23100.032.87888758
CGTCTAG49000.029.357121
GTGGTCG28400.025.75692754
CTACGGT46900.025.7462813
TTGACGG55400.024.4493531
TACGGTG49550.024.36933314
TCCGCAA50050.024.19569633
GACGCAC52200.024.0707746
GCCGACC41100.023.41899170
AGCGATA23700.023.33383270
GCGATCT466600.023.32133170
CCTACGG52400.023.24428612
CTAGACG55300.023.1644543
ACGCACC53550.023.0717937
GATCTCG35000.022.09990147
ACGGTGA55350.022.06866515
Produced by FastQC (version 0.11.5)