FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n02_mb143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n02_mb143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31807094
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA12700.035.00014559
CGACGAC20150.033.17673570
CGTATCA15350.029.86987762
CGTCTAG35750.028.098021
TCGCCGT16450.027.6596958
CCGTATC17050.026.8916561
CTACGGT35100.026.62387513
TACGGTG36800.025.2038314
GACGCAC40350.023.1597296
CTAGACG41500.023.1083133
TCCGCAA38800.023.00245733
CCTACGG40300.022.84114312
GCGATCT265800.022.59631570
TTGACGG42650.021.9928531
GCCGACC29200.021.93529970
ACGGTGA42550.021.55113815
ACGCACC43450.021.5073687
GTCCGCA42750.020.87708332
GTGGTCG21850.020.6635454
TGACGGC46800.020.41656132
Produced by FastQC (version 0.11.5)