FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n02_mb142.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n02_mb142.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31660047
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC32950.043.33939770
CGCCGTA15300.042.09161459
CGTATCA18750.034.53348562
TCGCCGT19450.033.29057758
CCGTATC20350.032.3342961
GCCGACC45850.031.4511170
CGTCTAG33350.027.3913351
CTACGGT28750.024.46947713
GTGGTCG26900.024.33076754
TACGGTG30300.023.91080514
GCGATCT270200.022.79845870
GATCTCG29150.022.21261247
GTCGCCG30500.021.57369257
TTGACGG40050.020.88631631
CTAGACG35350.020.7920043
AGCGATC385150.020.36526570
AGCGATA17550.020.34235670
CCTACGG35650.020.12615412
ATCTCGG32600.019.8618948
CGGTGGT33600.019.6874352
Produced by FastQC (version 0.11.5)