FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32914611
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3158160.9595009340988414No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG11400.025.7483481
CTACGGT14100.021.72859613
TACGGTG14150.021.65155414
ACCGATC9600.019.3011734
CTAGACG16900.018.9547793
CCTACGG16850.018.80243912
TCCGCAA17000.018.30254233
GGTACGA12800.017.9510118
ACGGTGA17800.017.79826415
CGATCTC10100.017.66318336
CGCTACG27800.017.44870670
GTACGAG13500.017.02016419
ACGCACC19200.016.5016527
CGCACCT19900.015.7459488
TACGAGG15300.015.70132620
ATCATCG13400.015.63332730
CATCGTT13400.014.8651832
GCTACGC23250.014.77189570
TAAGGCG15450.014.66692170
GACGGCT17650.014.657413533
Produced by FastQC (version 0.11.5)