FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n01_mb153.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n01_mb153.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22906007
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG26800.026.6426031
CTAGACG24950.021.4639553
CTACGGT24850.020.84533913
TACGGTG25400.020.39387514
TAGACGC41050.019.9523224
CGTAGAG171400.019.60313870
TCCGCAA25800.019.39905233
TTGACGG32900.019.04238531
GACGCAC28450.018.7005716
ACGCACC28350.018.5192857
GTCCGCA28200.018.24451832
CCTACGG28750.017.89600212
TGACGGC35800.017.69537532
ACGGTGA29800.017.6173715
GACGGCT37450.016.35499433
CGCACCT32200.016.1962538
TGCGACC22050.016.19086310
ACGTAGA221950.015.62728970
CGACCAA21750.015.44857812
CCGCAAA34850.015.06442934
Produced by FastQC (version 0.11.5)