FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n01_mb152.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n01_mb152.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28251737
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG29350.028.9784931
TTGACGG38750.020.86427331
CTAGACG33000.020.6828963
CTACGGT31350.020.65432513
TACGGTG32550.019.89280514
TAGACGC58150.019.3820924
TCCGCAA34050.019.01593233
GACGCAC35350.018.8131946
CAAGAGG452000.018.59169670
CCTACGG35350.018.41627912
TGACGGC45000.018.1997932
ACGCACC36800.018.0716257
GACGGCT44550.017.75512533
ATCTCGT39000.017.58963470
GTCCGCA36550.017.52373532
CGGCTAT54700.016.95592735
ACGGTGA41450.016.29684315
TCTCGTA26400.016.1741470
AACTGCG29650.016.0548297
GGTACGA29700.015.90904718
Produced by FastQC (version 0.11.5)