FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n01_mb151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n01_mb151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26425651
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG32400.026.7909681
CTAGACG36750.023.6205523
CTACGGT35600.023.30094113
TACGGTG36050.023.00999614
TTGACGG37300.022.3321731
TCCGCAA37500.022.30639833
GACGCAC38150.022.1118766
ACGCACC38200.021.9910137
CCTACGG39800.020.9301112
GTCCGCA40150.020.57260332
TGACGGC41850.020.32233632
TAGACGC66050.020.190694
GACGGCT42050.019.80951333
CCGAGGC324550.019.3465970
ACGGTGA44100.019.12700715
CGCACCT44050.019.0702368
AACTGCG30700.017.6722017
TCTCGTA25950.017.66841370
TATGCCG19850.017.63202949
TGCGACC27750.017.53208210
Produced by FastQC (version 0.11.5)