FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n01_mb150.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n01_mb150.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37006973
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC403420.10901188811092438No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG44950.030.6795351
CTACGGT47250.025.03761913
TACGGTG49600.023.63940614
CTAGACG51750.023.6052443
CGCTACG458300.022.56695270
TTGACGG58700.022.29953631
GACGCAC54850.022.271616
ACGCACC52650.022.2712787
CCTACGG54000.021.64882712
TCCGCAA54900.021.03797533
TAGACGC87400.020.9454154
ACGGTGA58950.020.1866615
GCTACGC317450.020.06602570
GTCCGCA58850.019.80432732
CGCACCT60800.019.6310028
TGACGGC67250.019.41238432
GACGGCT69550.018.51880633
CGGCTAT78850.017.97693635
TGCGACC36400.017.50062210
AACTGCG41400.017.4166877
Produced by FastQC (version 0.11.5)