FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n01_mb149.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n01_mb149.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30972107
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC336240.1085621975928212No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG35800.031.4815851
CTAGACG37950.024.9025293
CTACGGT38950.023.4536913
TCCGCAA39950.022.77819333
TACGGTG40200.022.72426214
TTGACGG48150.022.46078331
TAGACGC68100.021.3302174
ACGCACC43400.020.8077077
CCTACGG44100.020.79443712
GACGCAC45450.020.2545746
TGACGGC55650.019.74818632
GTCCGCA46500.019.41911732
GACGGCT56850.019.08507733
ACGGTGA47750.019.05769515
CGGCTAT63300.017.96976535
CGCACCT51200.017.7058898
CCGCAAA54200.017.0477634
GGTACGA33800.016.878618
GGGCGTT45150.015.73624133
CGAGACC1268300.015.62192670
Produced by FastQC (version 0.11.5)