FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n01_mb148.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n01_mb148.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29847651
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG34100.027.2004951
TATGCCG28650.022.72223749
TTGACGG41600.021.28579731
CTAGACG31150.019.6641983
TGCCGTC31400.019.61759451
CTCGTAT30500.019.50795745
CTACGGT30500.019.39395313
TGACGGC45950.019.3468832
GCCGTCT32400.019.12013852
TCCGCAA31450.019.02997633
CCGTCTT31900.018.98095953
GACGGCT47000.018.39339333
TACGGTG32400.018.14859414
ATGCCGT36950.017.8076350
TAGACGC54100.017.5336024
GACGCAC35350.017.2290136
CGAGACC1003600.017.1859270
CCTACGG34700.017.04658712
ACGCACC34400.016.8903477
GTCCGCA36800.016.54871232
Produced by FastQC (version 0.11.5)