FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n01_mb147.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n01_mb147.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25853413
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG28150.031.45751
CTACGGT28000.027.50030513
TACGGTG28800.026.61477314
GACGCAC31900.025.237346
CTAGACG31850.024.9468273
TCCGCAA30850.024.61882633
TTGACGG34250.024.32087531
ACGCACC32100.024.3163687
CCTACGG33250.023.47412512
GTCCGCA34650.022.4239732
CGCACCT36000.021.8762558
TGACGGC39300.021.28472932
ACGGTGA36200.021.1739815
TAGACGC57950.020.7177284
GACGGCT39700.020.27683333
GGGCGTT35450.018.95604533
ATCTCGT29550.018.95073770
CGGCTAT50600.018.8831735
TAGGGCG37200.017.78204331
GATCTCG32200.017.28243369
Produced by FastQC (version 0.11.5)