FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n01_mb146.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n01_mb146.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27574912
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG28950.032.6433561
CTACGGT31150.027.1920813
TACGGTG32500.025.63163614
ACGCACC33850.025.2300347
GACGCAC33700.025.1348826
TCCGCAA33600.024.47888433
CTAGACG35500.023.8600923
CCTACGG35800.023.26915412
TTGACGG39000.023.06383731
CGGACTC251700.022.99937870
GTCCGCA36700.022.50655432
AGACGGC34100.022.47778970
TGACGGC42200.021.3149232
ACGGTGA39250.021.2228815
AACTGCG33050.020.8632267
ACGGACT354550.020.75004670
CGCACCT40850.020.5642178
TGCGACC30300.020.09998310
GACGGCT44000.019.88613733
TAGACGC64050.019.7276944
Produced by FastQC (version 0.11.5)