FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n01_mb145.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n01_mb145.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21915071
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG23850.031.8457621
CTACGGT21800.027.13342313
TACGGTG22950.025.92618614
TTGACGG28950.025.50928931
ACGCACC25550.023.425487
CTAGACG25700.023.425163
GACGCAC25900.023.3796796
CCTACGG25900.022.9733612
TCCGCAA25200.022.91647733
TGACGGC32050.022.60511632
GACGGCT32950.022.09389933
ACGGTGA28050.021.46179215
GTCCGCA28100.021.17420232
CGCACCT29050.020.362028
TAGACGC47300.019.6839264
CGGCTAT43450.018.6074435
TGCGACC23300.018.02613610
AACTGCG25900.017.9736047
GCGACCA25000.016.94020811
CGAGACT691900.016.87513270
Produced by FastQC (version 0.11.5)