FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n01_mb144.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n01_mb144.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40656457
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG49350.032.554021
CTACGGT48850.026.94022813
TTGACGG60000.025.78303531
TACGGTG51500.025.62188314
CTAGACG55050.025.1147883
TCCGCAA52550.024.7761233
GACGCAC53950.024.6541426
ACGCACC54150.024.17487
TGACGGC65600.023.84881232
CCTACGG55550.023.6280812
GACGGCT67800.022.40387233
GTCCGCA58400.022.35419332
ACGGTGA60700.021.96885315
CGCACCT62600.020.9115378
TAGACGC103150.020.2920284
TGCGACC45800.020.17523410
AACTGCG49700.019.5784447
CGTCTTA45500.019.15397815
ATCATCG46500.019.11806130
GCGACCA48400.018.72969811
Produced by FastQC (version 0.11.5)