FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n01_mb143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n01_mb143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31807094
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG34450.032.2077331
CTACGGT34450.026.11085113
TACGGTG36950.024.34406514
TTGACGG44650.024.22139531
CTAGACG39000.023.873423
GACGCAC40150.023.277286
TCCGCAA39500.022.77184333
ACGCACC40850.022.2778227
CCTACGG40950.022.13739212
GTCCGCA41950.021.60876532
TGACGGC50250.021.52209532
ACCGTAC371250.020.5708670
CGCACCT45000.020.534288
ACGGTGA44750.020.49159815
GACGGCT52700.019.79100233
TAGACGC77000.019.7287434
GGGCGTT42300.019.4441833
TATGCCG25200.019.30529249
TGCCGTC25450.018.97813251
ATCTCGT42400.018.7380470
Produced by FastQC (version 0.11.5)