FastQCFastQC Report
Fri 19 Oct 2018
HMML7BGX7_n01_mb142.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMML7BGX7_n01_mb142.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31660047
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG31550.028.5112381
CTAGACG35250.025.4195963
CTACGGT34000.025.01501513
TACGGTG34550.024.51541914
GACGCAC36600.023.8130866
TCCGCAA36100.023.55932633
ACGCACC36650.023.3982477
TAGACGC68050.021.0886274
GTCCGCA40700.021.06858832
TTGACGG38850.020.90069631
CCTACGG40750.020.87155312
ACGGTGA42700.019.91793315
CGCACCT45350.018.6005658
TAAGGCG185900.018.431870
TTTCGAC29000.018.34464648
CGTCTAA33550.018.2569331
TGACGGC46150.018.0496632
CGAGACT1299850.017.78190470
GACGGCT45600.017.65333633
GAGACTA1029150.017.37147370
Produced by FastQC (version 0.11.5)