FastQCFastQC Report
Thu 2 Nov 2017
HMMGYBGX3_n02_atacseq_1000_5_ctr_root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMGYBGX3_n02_atacseq_1000_5_ctr_root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88385120
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG68294297.726899052691222No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT247500.018.06062156
CGCCGTA255500.017.57612255
CCGTATC275500.016.31304257
TGGTCGC273900.016.19214451
CGTATCA281150.015.71408558
GGTCGCC299050.014.94574452
GTGTATG951200.014.4593721
AGTGTAG1608650.013.54702369
GAGTGTA1955500.013.34227969
TCGCCGT358100.012.61731854
TGTATGA1056600.012.3301422
GGTGGTC376100.012.21336949
TGAGTAT980350.012.14443510
GTCGCCG395900.011.74252753
AAACCGC843450.011.67139225
TCGGTGG400100.011.56761147
TAGACCG142750.011.4937955
GAGTATA1018400.011.46061111
GTATGAT1074600.011.4099053
ACCGCAA837900.011.3670727
Produced by FastQC (version 0.11.2)