FastQCFastQC Report
Thu 2 Nov 2017
HMMGYBGX3_n02_atacseq_1000_4_ctr_root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMGYBGX3_n02_atacseq_1000_4_ctr_root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70426440
Sequences flagged as poor quality0
Sequence length75
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG27532373.9093797727103627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG1349250.041.46448569
CTCGGTG3148950.038.57567669
TCTCGGT4678050.025.9687268
ATCTCGG6043300.020.12269867
GATCTCG7317550.016.64677466
AGATCTC7819900.015.562860565
TAGATCT8849200.013.75267464
GTAGATA84750.013.24006469
GTAGATC10120600.012.01019963
CGGTGGT642700.010.80314569
GTCCTAA35750.010.6976121
TGTAGAT11455900.010.62660162
GTCTAAC29950.010.4685011
AGTGTAG15127800.09.2654169
GTGTAGA13172150.09.23883561
GTACTAG18650.09.2370041
ATTCTAA21250.09.0796481
GTGTAAC49450.09.057681
TCCTACA59750.08.9965372
TACAAGA117900.08.9724962
Produced by FastQC (version 0.11.2)