FastQCFastQC Report
Thu 2 Nov 2017
HMMGYBGX3_n02_atacseq_1000_2_ctr_root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMGYBGX3_n02_atacseq_1000_2_ctr_root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47014308
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14094822.9979852090984727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGTGG775600.040.9846469
CTCGGTG1652650.035.68814569
TCTCGGT2349650.025.10252668
ATCTCGG2993050.019.71400367
GATCTCG3685900.016.05320766
AGATCTC3954900.014.94444765
TAGATCT4524650.013.05582164
GTAGATC5241200.011.24377163
GTCTAAC32350.010.86344051
ATACTAA25700.010.5909631
GTCCTAC54100.010.2534511
GTGCTAA42150.010.05423451
TGTAGAT5984250.09.86736362
AGTGTAG8012000.09.73196969
CTATAGT32300.09.6036834
GTGTAGA6925800.09.50003769
GTATAAT22450.09.3617061
CTACACA41750.09.2461164
GTATTAA38450.09.22958851
TATACCG57850.08.9972695
Produced by FastQC (version 0.11.2)