FastQCFastQC Report
Thu 2 Nov 2017
HMMGYBGX3_n02_atacseq_1000_1_ctr_root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMGYBGX3_n02_atacseq_1000_1_ctr_root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63740934
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG9720451.5249933425826487No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTG2342900.037.264469
TCGGTGG1020600.036.56481669
TCTCGGT3361500.025.94004868
ATCTCGG4334900.020.1244367
GATCTCG5307950.016.46839166
AGATCTC5538400.015.75718265
TAGATCT6272400.013.92463164
GTAGATC7171600.012.15309663
TGTAGAT8147250.010.70517662
GTCCTAA40950.010.0966561
GTGTATG107800.09.9720871
AGTATAA74700.09.87848512
AGTGTAG10894950.09.87618569
GTACTAA22050.09.844241
GTGTAGA9408450.09.29355669
GAGTGTA12509700.09.21295469
GAGTATA76950.09.18593411
TGTATGA108200.08.82142
GTAGATA80300.08.77097969
TATGATT108650.08.723824
Produced by FastQC (version 0.11.2)