FastQCFastQC Report
Thu 2 Nov 2017
HMMGYBGX3_n01_atacseq_1000_5_ctr_root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMGYBGX3_n01_atacseq_1000_5_ctr_root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88385120
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG60956246.896663148729107RNA PCR Primer, Index 17 (96% over 28bp)
TCTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG1628740.18427762501199296TruSeq Adapter, Index 12 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG6708900.065.7150169
TATGCCG7197700.061.86655443
ATGCCGT7211900.061.78839544
GCCGTCT7210850.061.75504746
CGTATGC7216800.061.7548141
AAAGGGG7138800.061.74038368
GTATGCC7216650.061.73116342
TGCCGTC7227900.061.59656545
TCGTATG7259750.061.3922940
CTCGTAT7242250.061.16535639
CCGTCTT7318400.060.8562247
CTGCTTG7338750.060.73011854
TCTCGTA7306400.060.60183738
ATCTCGT7360600.060.1764837
CGTCTTC7436250.059.9320648
AATCTCG7420750.059.7043536
CTTCTGC7469200.059.68769551
TGCTTGA7485400.059.57475355
GCTTGAA7505600.059.4548856
AAAAGGG7434450.059.3546667