FastQCFastQC Report
Thu 2 Nov 2017
HMMGYBGX3_n01_atacseq_1000_4_ctr_root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMGYBGX3_n01_atacseq_1000_4_ctr_root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70426440
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG18816662.671817573059209RNA PCR Primer, Index 46 (96% over 28bp)
TCTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG3260330.4629411908368504TruSeq Adapter, Index 4 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG2191900.062.78928469
AAAGGGG2606050.052.75616568
AAAAGGG2672650.051.4930267
AAAAAAG2720050.050.96015565
AAAAAGG2715100.050.77929366
TTGAAAA2757200.050.61692458
TCGTATG2755050.050.46059440
GTATGCC2770150.050.2085642
GAAAAAA2791450.050.20536460
CTTGAAA2778200.050.18755357
CGTATGC2775600.050.09395241
TGAAAAA2806250.049.833759
CTCGTAT2764400.049.78449239
GTCTTCT2833850.049.05037749
TATGCCG2849500.048.83960343
TGCTTGA2861900.048.6341355
CCGTCTT2867800.048.4989947
TCTGCTT2870800.048.4617653
GCTTGAA2879250.048.3838656
TCTCGTA2917350.047.14550838
Produced by FastQC (version 0.11.2)