FastQCFastQC Report
Thu 2 Nov 2017
HMMGYBGX3_n01_atacseq_1000_2_ctr_root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMGYBGX3_n01_atacseq_1000_2_ctr_root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47014308
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG9951442.116683287138885TruSeq Adapter, Index 11 (96% over 28bp)
TCTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG1213850.25818735862282605TruSeq Adapter, Index 11 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG1193550.060.85301269
AAAGGGG1372000.052.92007468
TCGTATG1421850.051.90645640
CGTATGC1431350.051.53302441
AAAAGGG1416450.051.36649767
GTATGCC1434050.051.33621642
CTCGTAT1434550.051.1030439
CTTGAAA1466600.050.2952857
TTGAAAA1468950.050.2756758
AAAAAGG1453100.050.2145366
TATGCCG1471150.050.06617443
AAAAAAG1467750.049.90078765
CCGTCTT1490600.049.37304747
GAAAAAA1498400.049.36189360
TGAAAAA1507050.049.0398659
GTCTTCT1509500.048.8605449
TGCTTGA1510300.048.80272755
GCTTGAA1512700.048.75458556
TCTGCTT1524750.048.31908453
TCTCGTA1520550.048.12511438
Produced by FastQC (version 0.11.2)