FastQCFastQC Report
Thu 2 Nov 2017
HMMGYBGX3_n01_atacseq_1000_1_ctr_root.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMMGYBGX3_n01_atacseq_1000_1_ctr_root.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63740934
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG5895090.924851524767428RNA PCR Primer, Index 26 (100% over 29bp)
TCTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGG957730.15025352468164335RNA PCR Primer, Index 26 (96% over 31bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG783500.058.13464469
AAAGGGG917450.049.58275668
TCGTATG947200.048.24794440
AAAAGGG959100.047.42222667
CGTATGC967050.047.4076841
GTATGCC971600.047.23279642
CTCGTAT988900.046.12951739
AAAAAGG1017000.044.8394266
AAAAAAG1022900.044.77226665
TATGCCG1025450.044.69748343
CTTGAAA1035400.044.35500757
TTGAAAA1047750.043.9138958
GAAAAAA1069500.043.3704160
TGAAAAA1067150.043.35933759
CCGTCTT1066900.042.9449847
TCTGCTT1069100.042.76526353
TGCTTGA1076200.042.66517655
GCTTGAA1089750.042.18180556
TCTCGTA1086100.041.95762638
GTCTTCT1094400.041.61734849
Produced by FastQC (version 0.11.2)