FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8686802
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95739611.021271119106894No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC688670.7927773650187951Illumina Single End PCR Primer 1 (100% over 50bp)
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG152240.17525436863876948No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG139550.064.4837218
CGAGTCC20000.064.1668917
TAACTGA141500.063.2948232
AGCACAC141250.063.17819217
AGAGCAC176850.063.0347515
TTAGCTA87550.062.75888419
CACGCGT18500.062.57652319
GCACGCG26950.062.55015618
GCTAAAC62300.062.4510722
CACACGT98950.062.42090619
ACGAGTC20850.061.86998716
ACACGTC61050.061.6484120
ACTGAAG144250.061.4612734
CGCGTCT12650.060.84222821
AGATCAT151050.060.815139
CGCGCGT24300.060.7381219
CACGTAG29050.060.2139121
GATCATC74450.060.21035810
TAGCTAA92700.059.8437420
GTCCTCT29000.059.58325220
Produced by FastQC (version 0.11.5)