FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n02_776-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n02_776-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8338158
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1217891.4606223580795663No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC711190.8529341852241227Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG122900.046.1386767
AAAGGGG124150.045.78724766
CGCCGTA136900.045.6003247
CCGTATC139400.044.6880249
GCCGTAT142650.043.59105748
TCGCCGT142150.043.49742546
GGTCGCC144500.043.12750644
GTCGCCG144450.043.11976245
CGTATCA147850.042.23069450
GTGGTCG151500.041.2001542
CTCGGTG148750.040.9455138
AGGGGGG140850.040.7309568
ATCTCGG151150.040.15649836
TCTCGGT152150.039.93831637
GATCTCG153300.039.27665335
TGGTCGC158500.039.20516643
TCGGTGG163300.037.8759239
GGTGGTC164400.037.8375641
AGATCTC162600.037.58963434
GTATCAT165600.037.19915451