FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n02_1566.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n02_1566.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9301418
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2756062.9630535903235398Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1419001.5255738426119543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG404000.053.37207867
AAAGGGG406000.053.23885766
AGGGGGG424300.051.01696868
CACACGA43550.047.8780319
GCACACG75250.047.14116318
AAAAGGG466050.046.7032765
GAGCACA131200.045.22339616
AGAGCAC138650.045.01384415
ACACGAG46600.044.36900720
CACGAGA48050.043.25456621
AAGAGCA219000.041.82121714
AGCACAC96700.041.0620417
CACGTAG22200.034.6743721
GAGCAAG64550.033.11433816
ACGAGAT67600.030.9005722
CACACGT43500.030.8026219
GCACAAG49950.029.83615918
GGTCGGA22000.029.3223652
AAAAAGG752350.029.27063264
ACACGTA28350.029.2464720
Produced by FastQC (version 0.11.5)