FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n02_1565.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n02_1565.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5615982
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC678401.2079810797114379Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG633851.1286539023807411No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG30400.049.4865818
GAGCACA58250.048.05010616
AAGGGGG117250.046.6023167
AGAGCAC61500.046.5917615
CACACGA18300.046.4566419
AAAGGGG122500.045.14793466
AGGGGGG129000.042.9269668
AAGAGCA107400.040.5573214
AGCACAC42050.040.4358617
AAAAGGG147400.037.63898565
CACGAGA22350.037.41617621
CACACGT17100.036.41803419
ACACGAG24300.034.69791420
GAGCAAG33950.033.2861116
CACGTAG11600.031.36998721
GCACAAG27850.031.02875318
AGCACAA31600.028.01108217
GGCGGTC7700.027.7823851
CCGGGTG3950.027.522951
ACGAGAT30750.027.42274522
Produced by FastQC (version 0.11.5)