FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n02_1564.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n02_1564.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8382769
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1114911.3300020554067518Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG873341.041827587041943No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG60600.053.28362318
GAGCACA113700.050.67516716
AGAGCAC119000.050.0056915
CACACGA35750.048.63543319
AAGGGGG182750.048.58836767
AAAGGGG190700.046.61761566
AGCACAC76500.046.4161717
AAGAGCA200450.045.7256514
AGGGGGG196350.045.45403768
CACGAGA38700.043.939221
ACACGAG41350.041.28732720
CACACGT35400.040.7161219
GAGCAAG61050.040.16931516
AAAAGGG229150.039.1771465
CACGTAG22500.036.23266221
GCACAAG50250.035.71461518
AGAGCAA72850.034.6712715
ACACGTA26600.033.27448320
AGCACAA56400.031.88222917
ACGAGAT56750.030.45705622
Produced by FastQC (version 0.11.5)