FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n02_1561.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n02_1561.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7852811
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG923561.1760884096153594No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC772280.9834440176899711Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG34950.048.0485318
GAGCACA68400.047.56808516
AGAGCAC74300.045.7684615
AAAGGGG135250.045.55197566
AGCACAC45350.043.66444817
AAGGGGG141050.043.6537667
AGGGGGG152750.040.79002868
CACACGA23550.040.11108819
AAGAGCA144900.036.31351514
ACACGAG25450.036.29127520
AAAAGGG173500.035.7515665
CACGAGA26200.035.6557221
CACACGT21950.032.35587319
GAGCAAG45100.030.87417416
CACGTAG15100.027.80504221
GGGGGTA22350.027.6247431
GGGGGAT18550.025.908351
GGCGGTC9100.025.828341
ACACGTA17500.025.7891920
GGGCGTA10900.025.7469981
Produced by FastQC (version 0.11.5)