FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n02_1560.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n02_1560.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7489671
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2154192.876214455881974Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG751621.0035420781500282No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG306400.054.64063667
AAAGGGG309800.054.08546466
GCACACG53550.052.9832918
AGGGGGG322900.052.2929168
CACACGA34350.051.6374319
GAGCACA101200.049.5734416
AGAGCAC105600.049.39627515
AAAAGGG344250.049.079365
CACGAGA38750.045.59929721
AAGAGCA176350.045.42976414
AGCACAC69000.045.0744317
ACACGAG39750.044.6225320
GAGCAAG54350.039.2654716
CACACGT27150.038.14215519
AAAAAGG456650.037.45142464
GCACAAG40850.037.16851818
ACACGTA19550.034.8956420
CACGTAG18700.034.4282321
AGAGCAA64700.034.0115815
AGCACAA46350.032.9089717
Produced by FastQC (version 0.11.5)