FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n02_1559.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n02_1559.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10042074
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1757481.7501165595871928Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1372371.3666200826641985No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG77050.052.9423918
GAGCACA143150.052.27614216
AGAGCAC150400.051.5007915
CACACGA47100.051.40073419
AAAGGGG262750.051.33873766
AAGGGGG265000.050.9559767
AGCACAC93600.047.95511217
AAGAGCA250850.047.879114
AGGGGGG287050.047.49300868
ACACGAG52300.046.08923320
CACGAGA52900.045.90149321
AAAAGGG312750.043.43285465
GAGCAAG77000.042.57287616
CACACGT40850.038.11115319
GCACAAG63500.037.73935318
CACGTAG25600.036.49172221
AGAGCAA93500.035.77094315
AGCACAA71450.034.07933417
ACGAGAT72700.033.49621622
ACACGTA29000.032.81352220
Produced by FastQC (version 0.11.5)