FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n02_1556.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n02_1556.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11136265
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2599232.334023121755813Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1025470.9208383600785361No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG115500.055.28007518
CACACGA73800.054.4697419
GAGCACA220950.052.2043716
CACGAGA77300.051.46565621
AGAGCAC230250.051.09884315
AAAGGGG397600.050.4584966
AAGAGCA365300.049.75327314
AAGGGGG405050.049.32266267
ACACGAG81500.048.72253820
GAGCAAG102550.048.2387116
AGCACAC147900.047.68811417
AGGGGGG424700.047.164168
AAAAGGG451100.044.76900565
CACGTAG35550.044.09353621
GCACAAG86150.041.9500818
ACACGTA38500.041.71032720
AGAGCAA122250.041.55292515
CACACGT55050.041.49994719
AGCACAA91250.040.06555617
ACGAGAT100350.039.7135722
Produced by FastQC (version 0.11.5)