FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n02_1547.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n02_1547.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7492201
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2108272.8139528023874427Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG821351.0962733114074223No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC210100.058.2785515
GAGCACA206200.058.2101616
GCACACG107150.056.90857718
CACACGA70700.055.2736219
AGCACAC125950.054.7189417
AAGAGCA360350.054.6964414
AAAGGGG294350.053.7243266
AAGGGGG294700.053.6241567
ACACGAG72800.053.05444720
CACGAGA73650.052.39883821
GAGCAAG110700.051.98583216
AGGGGGG307350.051.72440368
GCACAAG82600.050.48574418
AAAAGGG326650.048.74431665
AGAGCAA126300.046.58963815
AGCACAA92250.045.6593517
CACGTAG33400.043.78735421
AGCAAGA134750.042.7596817
ACGAGAT91650.042.26016222
ACACGTA36050.042.21512620
Produced by FastQC (version 0.11.5)