FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n02_1543.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n02_1543.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8555433
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3637274.251415445600474Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1061171.2403463389871674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG133950.058.08447618
CACACGA86900.057.4489919
AAGAGCA382750.056.03003714
AAGGGGG483850.056.00378867
GAGCACA249500.055.91911316
AGAGCAC256350.055.80324615
AAAGGGG489950.055.4137766
CACGAGA89450.055.23105621
AGGGGGG503550.054.01389368
ACACGAG92400.053.6886520
GAGCAAG106450.052.35350416
AGCACAC162450.052.3316917
AAAAGGG529350.051.54733765
GCACAAG86150.050.06979818
AGCACAA89050.048.91179317
CACGTAG38700.048.6407521
AGAGCAA120850.047.21532415
CACACGT57050.046.8506819
ACACGTA41800.046.11620320
ACGAGAT109350.044.92309622
Produced by FastQC (version 0.11.5)