FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n02_1215a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n02_1215a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9583762
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC4615454.8159063215468Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1424501.4863682967085368No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC100260.1046144509849055Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG604650.057.6234567
AAAGGGG609000.057.34929366
AGGGGGG630900.055.56925268
GCACACG86600.054.90123418
AGAGCAC153500.054.05993315
GAGCACA149150.053.87743816
AAAAGGG658650.053.27588765
AGCACAC100250.052.0315817
CACACGA54000.051.8294719
ACACGAG54100.050.95766420
AAGAGCA256100.047.5380214
AAAAAGG747050.047.4217664
CACGAGA58650.047.12889521
CATTAAA876500.044.1213555
CACACGT42750.042.88206519
AAAAAAG848050.042.83547263
ATCATTA897750.042.6740853
TCATTAA899100.042.6310354
GTATCAT890850.042.6278951
ATTAAAA937450.041.94645756
Produced by FastQC (version 0.11.5)