FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n02_1214-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n02_1214-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13003847
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC5499184.228887036274727Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2023911.5563932734674593No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC136820.10521501829420171Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG728550.057.1156866
AAGGGGG730100.056.8788567
AGGGGGG759000.054.97570868
AAAAGGG794100.052.61370565
GCACACG84450.051.8254518
GAGCACA151500.051.28826516
AGAGCAC156450.050.9848815
AGCACAC103950.047.5892317
CACACGA54150.047.22888619
ACACGAG53850.046.51729220
AAAAAGG913000.046.18380464
CACGAGA59350.042.7424621
CATTAAA1101650.042.0413155
AAGAGCA276200.041.43245314
AAAAAAG1043200.041.3631163
GTATCAT1118250.040.6797651
TCATTAA1132650.040.49436654
ATCATTA1145300.040.0391453
CGTATCA1151050.039.98106850
CCGTATC1153950.039.7991349
Produced by FastQC (version 0.11.5)