FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n02_1204-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n02_1204-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6632847
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC3424475.162896113840708Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1216751.834430976622859No Hit
AGGTCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC70710.10660580592315788Illumina Single End PCR Primer 1 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG440950.057.957466
AAGGGGG446000.057.31685667
AGGGGGG463950.055.49896668
GCACACG54250.055.39396318
GAGCACA95100.054.4812216
AAAAGGG476800.053.84980865
AGAGCAC100100.053.57683615
CACACGA35650.050.83300819
AGCACAC65500.050.7935117
ACACGAG36700.049.09267820
AAAAAGG545900.047.53392464
CATTAAA614300.046.51245555
GTATCAT605950.046.3896751
AAGAGCA167000.046.27519614
CACGAGA38950.046.17217321
CGTATCA630850.045.2321350
CCGTATC629300.045.20801549
ATCATTA624900.045.17251653
TCATTAA625150.045.15649854
TATCATT638250.043.98884252
Produced by FastQC (version 0.11.5)