FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n02_1091-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n02_1091-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5370004
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2179184.058060291947641Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG959801.7873357263793472No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG284450.056.7634666
AAGGGGG292300.055.16713367
AGGGGGG305500.053.1847468
GCACACG31050.052.84495518
AAAAGGG309250.052.3364465
AGAGCAC53800.050.20319715
GAGCACA52100.049.7595916
CACACGA17950.048.92215719
AGCACAC37100.048.28279517
AAAAAGG352950.046.3625864
ACACGAG19000.044.92960420
AAAAAAG407850.041.2124363
CACACGT17350.040.73317319
CATTAAA445800.040.26046855
AAGAGCA98150.039.0675914
ATCATTA456250.038.86585653
GTATCAT453550.038.7672551
ATTAAAA472600.038.66476456
TCATTAA459650.038.6403954
CCGTATC463900.038.29688649
Produced by FastQC (version 0.11.5)