FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8686802
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACA1158371.3334826786658658TruSeq Adapter, Index 14 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG844220.9718421117460718No Hit
AGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAACT96590.11119166754347573TruSeq Adapter, Index 14 (96% over 29bp)
GAGCACACGTCTGAACTCCAGTCACAGATCGGAAGAGCACACGTCTGAAC96130.11066212859461974TruSeq Adapter, Index 14 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGACTG49050.050.9799961
TGACTGG51350.048.753822
ACTGGAG58750.042.910414
GGAGTTC63400.040.7925347
AAAAAGG166250.036.33411870
TGGAGTT74350.034.990566
GACTGGA80150.031.8902113
GCTTGAA186300.031.41061460
AGCTCGG33000.030.65045441
GAGTTCA83750.030.5144868
TCACAGA314900.029.88571531
CTTGAAA206000.029.17098661
GTCACAG312550.029.14725730
CTGGAGT91750.028.5447395
GCTCGGA27900.028.47571842
CACAGAT342750.028.35609432
GCTCGGG9050.027.84434142
AAAAGGG57950.027.72009770
ATGCCGG26100.026.54973470
CTGCTTG213150.026.23882958
Produced by FastQC (version 0.11.5)