FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n01_776-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n01_776-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8338158
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATG772480.9264396285126764TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG138800.045.03876558
GCCGTCT140850.044.50403650
TGCCGTC144050.043.58828749
CCGTCTT147900.042.47730351
TGCTTGA149250.042.0025659
TATGCCG149900.041.9098147
ATGCCGT151100.041.6472148
GCTTGAA156600.040.32174360
GTATGCC156400.040.23516546
GATCTCG154750.039.3069540
CTCGTAT160600.038.8124643
CGTAGAG162900.038.52112634
AGTCACG166750.038.09324629
CGTATGC165650.038.03066345
CACGTAG166150.037.99933632
ATCTCGT161500.037.75077441
GTCACGT168300.037.61765330
CGTCTTC167600.037.58885652
AGATCTC164850.036.87680439
TCGTATG170450.036.8364944