FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n01_1566.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n01_1566.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9301418
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG2708182.9115775680654283TruSeq Adapter, Index 7 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCGCGTATG148760.15993260382449215TruSeq Adapter, Index 7 (97% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG140020.1505361870630908No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG442150.049.5881470
AAAAAAG578750.038.91261369
GCTTGAA671050.031.9181460
GCCGGCT44000.031.66212556
CGTGTGC85600.031.3335421
TGCTTGA669650.031.02291159
CTGCTTG677400.030.45095458
CTTGAAA715550.030.29009661
CCGGCTT50200.029.91322757
TCTGCTT727350.028.74957857
GTGTGCT95350.028.1969132
CGGCTTC50850.028.15412558
TTCTGCT715250.027.93906456
GCGTGAA63900.027.93664766
TTGAAAA785700.027.901762
CTTCTGC749300.027.36549455
TGAAAAA849600.027.20393863
GTCTTCT745850.026.9689753
CCGTCTT777550.026.78310851
CGTCTTC772250.026.6362152
Produced by FastQC (version 0.11.5)