FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n01_1565.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n01_1565.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5615982
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG777591.3846020161745531TruSeq Adapter, Index 6 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG158200.036.06292770
CGTGTGC28800.034.7705841
GCTTGAA170450.034.150960
CTGCTTG178800.032.36028358
TGCTTGA180600.032.1152859
ATGCCGG12300.029.3097470
CTTGAAA201750.029.19937961
ACGTGTG15250.028.2405871
TCTGCTT207400.028.03288557
CCGTCTT209700.027.73939351
CTTCTGC210050.027.51208955
GTGTGCT36500.027.4300442
CGTCTTC214150.027.08125952
TTCTGCT215400.026.71548556
GTCTTCT217900.026.59937153
GCCGTCT219200.026.5052550
AAAAAAG228850.025.38872569
TGCCGTC230900.025.29861649
TCTTCTG229050.025.24520754
ATGCCGT243850.024.04121848
Produced by FastQC (version 0.11.5)