FastQCFastQC Report
Fri 2 Aug 2019
HMM52AFXY_n01_1564.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHMM52AFXY_n01_1564.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8382769
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATG1061121.2658347140425794TruSeq Adapter, Index 5 (100% over 49bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATG93270.11126395108823828TruSeq Adapter, Index 5 (97% over 49bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAAGG245300.037.94080770
CGTGTGC58450.034.207911
GCTTGAA270050.033.85618660
TGCTTGA281950.031.80670459
CTGCTTG282700.031.61088458
ACGTGTG31300.030.3179511
CTTGAAA310300.029.84759761
ATGCCGG40000.029.48870370
GTGTGCT69750.028.8599012
TCTGCTT326000.027.8095457
TTCTGCT334800.026.40898956
AAAAAAG362250.026.3389269
CTTCTGC344600.026.13519755
GTCTTCT355250.025.19314453
CCGTCTT362750.025.18290751
CGTCTTC361800.025.09469652
TTGAAAA382400.024.63200262
GCCGTCT370550.024.32221850
TCTTCTG370200.024.24280554
TGAAAAA402750.024.09137363
Produced by FastQC (version 0.11.5)